Analysis of influenza A H3N2 strains isolated in England during 1995–1996 using polymerase chain reaction restriction
Identifieur interne : 001B94 ( Main/Exploration ); précédent : 001B93; suivant : 001B95Analysis of influenza A H3N2 strains isolated in England during 1995–1996 using polymerase chain reaction restriction
Auteurs : J. S. Ellis [Royaume-Uni] ; C. J. Sadler [Royaume-Uni] ; P. Laidler [Royaume-Uni] ; H. Rebelo De Andrade [Portugal] ; M. C. Zambon [Royaume-Uni]Source :
- Journal of Medical Virology [ 0146-6615 ] ; 1997-03.
English descriptors
- Teeft :
- Amino acid substitutions, Amplification, Antigenic, Antigenic analysis, Antigenic composition, Antigenic drift, Antigenic profile, Antigenic shift, Antigenic variation, Antigenically, Assay, Birmingham research unit, Blood cells, Cdna synthesis, Clinical indices, Clinical material, Coding, Coding changes, Elli, England influenza, England strains, Enzyme, Epidemic activity, Epidemic influenza activity, Epidemic period, February, General practitioners, Genetic, Genetic diversity, Genetic drift, Genetic variants, Genetic variation, Haemagglutinin, Health laboratory, Hpall, Influenza, Influenza activity, Influenza strains, Influenza vaccine, Influenza variants, Influenza virus, Influenza viruses, Insufficient material, Large number, Less reactivity, Major antigenic sites, Medical research, National institute, Oligonucleotide mapping, Pcrrestriction analysis, Phylogenetic analysis, Prototype viruses, Rapid analysis, Rcgp, Rcgp consultation population, Rcgp virological surveillance scheme, Receptor binding site, Respiratory virus laboratory, Restriction, Restriction analysis, Restriction endonuclease digestion, Restriction enzyme digestion, Restriction enzymes, Restriction influenza haemagglutinin, Royal college, Sequence analysis, Sequential influenza, Sequential strains, Significant antigenic drift, Similar antigenically, Sporadic influenza activity, Sufficient material, Surface antigens, Temporal alteration, Throat swabs, Vaccine, Vaccine strain, Variant, Virus.
Abstract
A polymerase chain reaction‐restriction (PCR‐restriction) endonuclease assay was developed to allow rapid analysis of influenza A H3N2 viruses circulating in England during 1995–1996. Restriction endonuclease digestion with two enzymes of amplicons derived from PCR of the HA1 portion of the influenza haemagglutinin (HA) gene was able to differentiate antigenically similar influenza strains into two groups. Group I variants were similar genetically to the 1995/96 vaccine strain, A/Johannesburg/33/94, whereas the HA sequences of Group II variants were similar genetically to the reference virus A/Thessaloniki/1/95. Of the 700 England A H3N2 strains isolated between February 1995 and the end of April 1996, 384 were analysed by this method. PCR‐restriction analysis of sequential influenza isolates revealed a temporal alteration in prevalence of the two variants. Groups I and II variants cocirculated with equal frequency during a period of sporadic influenza activity, but following the onset of epidemic influenza activity in 1995, only Group II variants were detected. PCR‐ restriction analysis was found to be a rapid method for studying genetic variation which could be applied to a large number of samples and provide information about the direction of genetic drift in the HA gene of influenza virus. J. Med. Virol. 51:234–241, 1997. © 1997 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/(SICI)1096-9071(199703)51:3<234::AID-JMV16>3.0.CO;2-K
Affiliations:
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Zambon</name><affiliation wicri:level="3"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Enteric and Respiratory Virus Laboratory, Central Public Health Laboratory, London</wicri:regionArea><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName></affiliation><affiliation wicri:level="1"><country xml:lang="fr" wicri:curation="lc">Royaume-Uni</country><wicri:regionArea>Correspondence address: Enteric and Respiratory Virus Laboratory, Central Public Health Laboratory, 61, Colindale Avenue, London NW9 5HT</wicri:regionArea><wicri:noRegion>London NW9 5HT</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Medical Virology</title><title level="j" type="alt">JOURNAL OF MEDICAL VIROLOGY</title><idno type="ISSN">0146-6615</idno><idno type="eISSN">1096-9071</idno><imprint><biblScope unit="vol">51</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="234">234</biblScope><biblScope unit="page" to="241">241</biblScope><biblScope unit="page-count">8</biblScope><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>New York</pubPlace><date type="published" when="1997-03">1997-03</date></imprint><idno type="ISSN">0146-6615</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0146-6615</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Amino acid substitutions</term><term>Amplification</term><term>Antigenic</term><term>Antigenic analysis</term><term>Antigenic composition</term><term>Antigenic drift</term><term>Antigenic profile</term><term>Antigenic shift</term><term>Antigenic variation</term><term>Antigenically</term><term>Assay</term><term>Birmingham research unit</term><term>Blood cells</term><term>Cdna synthesis</term><term>Clinical indices</term><term>Clinical material</term><term>Coding</term><term>Coding changes</term><term>Elli</term><term>England influenza</term><term>England strains</term><term>Enzyme</term><term>Epidemic activity</term><term>Epidemic influenza activity</term><term>Epidemic period</term><term>February</term><term>General practitioners</term><term>Genetic</term><term>Genetic diversity</term><term>Genetic drift</term><term>Genetic variants</term><term>Genetic variation</term><term>Haemagglutinin</term><term>Health laboratory</term><term>Hpall</term><term>Influenza</term><term>Influenza activity</term><term>Influenza strains</term><term>Influenza vaccine</term><term>Influenza variants</term><term>Influenza virus</term><term>Influenza viruses</term><term>Insufficient material</term><term>Large number</term><term>Less reactivity</term><term>Major antigenic sites</term><term>Medical research</term><term>National institute</term><term>Oligonucleotide mapping</term><term>Pcrrestriction analysis</term><term>Phylogenetic analysis</term><term>Prototype viruses</term><term>Rapid analysis</term><term>Rcgp</term><term>Rcgp consultation population</term><term>Rcgp virological surveillance scheme</term><term>Receptor binding site</term><term>Respiratory virus laboratory</term><term>Restriction</term><term>Restriction analysis</term><term>Restriction endonuclease digestion</term><term>Restriction enzyme digestion</term><term>Restriction enzymes</term><term>Restriction influenza haemagglutinin</term><term>Royal college</term><term>Sequence analysis</term><term>Sequential influenza</term><term>Sequential strains</term><term>Significant antigenic drift</term><term>Similar antigenically</term><term>Sporadic influenza activity</term><term>Sufficient material</term><term>Surface antigens</term><term>Temporal alteration</term><term>Throat swabs</term><term>Vaccine</term><term>Vaccine strain</term><term>Variant</term><term>Virus</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A polymerase chain reaction‐restriction (PCR‐restriction) endonuclease assay was developed to allow rapid analysis of influenza A H3N2 viruses circulating in England during 1995–1996. Restriction endonuclease digestion with two enzymes of amplicons derived from PCR of the HA1 portion of the influenza haemagglutinin (HA) gene was able to differentiate antigenically similar influenza strains into two groups. Group I variants were similar genetically to the 1995/96 vaccine strain, A/Johannesburg/33/94, whereas the HA sequences of Group II variants were similar genetically to the reference virus A/Thessaloniki/1/95. Of the 700 England A H3N2 strains isolated between February 1995 and the end of April 1996, 384 were analysed by this method. PCR‐restriction analysis of sequential influenza isolates revealed a temporal alteration in prevalence of the two variants. Groups I and II variants cocirculated with equal frequency during a period of sporadic influenza activity, but following the onset of epidemic influenza activity in 1995, only Group II variants were detected. PCR‐ restriction analysis was found to be a rapid method for studying genetic variation which could be applied to a large number of samples and provide information about the direction of genetic drift in the HA gene of influenza virus. J. Med. Virol. 51:234–241, 1997. © 1997 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>Portugal</li><li>Royaume-Uni</li></country><region><li>Angleterre</li><li>Grand Londres</li></region><settlement><li>Londres</li></settlement></list><tree><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Ellis, J S" sort="Ellis, J S" uniqKey="Ellis J" first="J. S." last="Ellis">J. S. Ellis</name></region><name sortKey="Laidler, P" sort="Laidler, P" uniqKey="Laidler P" first="P." last="Laidler">P. Laidler</name><name sortKey="Sadler, C J" sort="Sadler, C J" uniqKey="Sadler C" first="C. J." last="Sadler">C. J. Sadler</name><name sortKey="Zambon, M C" sort="Zambon, M C" uniqKey="Zambon M" first="M. C." last="Zambon">M. C. Zambon</name><name sortKey="Zambon, M C" sort="Zambon, M C" uniqKey="Zambon M" first="M. C." last="Zambon">M. C. Zambon</name></country><country name="Portugal"><noRegion><name sortKey="De Andrade, H Rebelo" sort="De Andrade, H Rebelo" uniqKey="De Andrade H" first="H. Rebelo" last="De Andrade">H. Rebelo De Andrade</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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